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Visualisation of secreted translocator and effector proteins during EPEC and EHEC 0157:H7 type III secretion

Reference	B17144
Principal Investigator / Supervisor	Dr Stuart Knutton
Co-Investigators / Co-Supervisors	Professor Gad Frankel
Institution	University of Birmingham
Department	Medical Sciences - Medicine
Funding type	Research
Value (£)	179,124
Status	Completed
Type	Research Grant
Start date	01/09/2002
End date	01/09/2005
Duration	36 months

Abstract

Enteropathogenic (EPEC) and enterohaemorrhagic E.coli (EHEC) employ a type III secretion system (TTSS) to translocate effector proteins into host intestinal cells. Electron microscopy combined with in situ immunogold labelling will be used to examine the structure of the EPEC:EHEC TTSS, its assembly and the secretion of effector proteins. Type III secreted proteins will be put under control of the tight arabinose promoter in order to study the kinetics of assembly and regulation of secretion. A recently developed red blood cell (RBC) model of infection combined with in situ gold labelling will be employed to examine cell contact-dependent type III secretion and formation of the host cell membrane translocation pore. The proposed studies should lead to a more detailed understanding of the structure and assembly of the EPEC:EHEC TTSS.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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