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Structural and functional studies on the focal adhesion proteins talin and vinculin

Reference	B16717
Principal Investigator / Supervisor	Professor David Critchley
Co-Investigators / Co-Supervisors	Dr Igor Barsukov, Professor Jonas Emsley, Professor Gordon Roberts
Institution	University of Leicester
Department	Biochemistry
Funding type	Research
Value (£)	359,004
Status	Completed
Type	Research Grant
Start date	01/06/2002
End date	31/10/2005
Duration	41 months

Abstract

Talin and vinculin are interacting cytoskeletal proteins which link the integrin family of adhesion molecules to F- actin, and we propose a multidisciplinary approach to study the structure and function of these proteins. We will determine structures (X-ray/NMR) of (i) the talin FERM domain alone and in complex with IP3 and integrin cytodomain peptides (ii) the talin rod repeats which contain vinculin-binding sites and for which we have crystals/NMR spectra (iii) the talin C-terminal actin-binding site. Vinculin projects include the talin-binding domain singly and in complex with either a talin peptide or the vinculin tail which masks the binding site. Structure/function data will come from analysing the ability of talin and vinculin mutants with inactivating mutations in ligand binding sites to rescue the adhesion/motility defects of talin and vinculin (-/-) cells.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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