BBSRC Portfolio Analyser
Award details
Rapid proteinase substrate mapping using positional scanning libraries
Reference
B16694
Principal Investigator / Supervisor
Professor Robin Leatherbarrow
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Chemistry
Funding type
Research
Value (£)
216,764
Status
Completed
Type
Research Grant
Start date
01/01/2002
End date
31/03/2005
Duration
39 months
Abstract
Combinatorial libraries will be created that will allow rapid determination of the substrate specificity of a proteinase enzyme. Two different types of positional scanning libraries will be created. The first will build on past work with proteinase inhibitors based on peptides derived from the reactive site of Bowman-Birk Inhibitor. These will permit inhibitory sequences to be defined, which in turn will provide information about preferred substrate requirements as these inhibitors are substrate mimics. The second approach will be to generate libraries of peptides that incorporate the fluorogenic amido methyl coumarin group. Iterative deconvolution of these libraries will permit optimum sequences to be defined.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search