BBSRC Portfolio Analyser
Award details
Protein binding at DNA ends: determining the specificity of multiple DNA binding sites within E. coli DNA ligase
Reference
B16634
Principal Investigator / Supervisor
Dr Richard Bowater
Co-Investigators /
Co-Supervisors
Professor Andrew Hemmings
Institution
University of East Anglia
Department
Biological Sciences
Funding type
Research
Value (£)
157,864
Status
Completed
Type
Research Grant
Start date
13/05/2002
End date
12/06/2004
Duration
25 months
Abstract
DNA ligases join breaks in the backbone of DNA. In a current BBSRC-funded study, we have demonstrated that the C-terminal BRCT domain of E. coli DNA ligase binds DNA. Building on this intriguing observation, we aim to further our biochemical and mutagenesis analysis of the E. coli DNA ligase. We now propose a programme of crystallographic and biophysical studies in combination with mutational analyses that will be used to determine the structural basis for the macromolecular interaction between DNA and domains within the E. coli DNA ligase. These important studies will delineate the DNA-binding function of the BRCT domain in the eubacterial enzyme, which may be relevant to its role in a wide range of DNA repair proteins.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search