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X-ray crystallographic studies of an echovirus- receptor complex

Reference	B16601
Principal Investigator / Supervisor	Professor Susan Lea
Co-Investigators / Co-Supervisors
Institution	University of Oxford
Department	Biochemistry
Funding type	Research
Value (£)	197,084
Status	Completed
Type	Research Grant
Start date	17/02/2003
End date	16/08/2005
Duration	30 months

Abstract

The interaction of a virus with its receptor is the first stage in entry of the virus to the host cell. Our understanding of these complexes is limited at the present time to low resolution structures determined using cryo-electron microscopy. This study aims to produce crystals containing a complex between a human picornavirus and its receptor and use these crystals to determine the structure at near-atomic resolution. Specifically, mutagenesis, informed by X-ray structures of the isolated components, cryo-electron microscopy of the complex and surface plasmon resonance studies will be used to determine a minimal fragment of Decay Accelerating Factor (DAF) capable of binding to Echovirus 11 (EV11). A complex between EV11 and DAF will then be targeted for near-atomic resolution structure determination using X-ray crystallography.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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