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Telomerase structure function studies using fluorescence and single molecule detection

ReferenceB16587
Principal Investigator / Supervisor Professor Sir Shankar Balasubramanian
Co-Investigators /
Co-Supervisors
Dr Mark Farrow, Professor David Klenerman
Institution University of Cambridge
DepartmentChemistry
Funding typeResearch
Value (£) 194,292
StatusCompleted
TypeResearch Grant
Start date 22/08/2002
End date 22/08/2005
Duration36 months

Abstract

The proposed work will employ FRET and single molecule detection to first study the structure and conformational dynamics of the RNA component of human telomerase (hTR) in native form and as its complex with the protein component of human telomerase. The initial strategy will use synthetic labelled PNA as specific hybridisation probes. We will then build on these studies and carry out experiments to observe catalysis by human telomerase using single molecule FRET.

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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