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Award details
Achieving photo-control of protein activity: application to bZIP and bHLH DNA binding proteins
Reference
B16000
Principal Investigator / Supervisor
Professor Rudolf Allemann
Co-Investigators /
Co-Supervisors
Institution
University of Birmingham
Department
School of Chemistry
Funding type
Research
Value (£)
194,180
Status
Completed
Type
Research Grant
Start date
01/12/2001
End date
31/05/2005
Duration
42 months
Abstract
The award holders have successfully designed, synthesised and characterised a peptide containing a photo- isomerisable cross-linker. Photo-isomerisation of the cross-linker leads to a change in the peptide conformation from random coil to alpha-helical. This work has already been extended to control the helicity of the leucine zipper peptide from GCNA4 (GCNA-ZIP), allowing photo-control of dimerization. We seek to extend this technology by application to two DNA binding proteins. The lessons already learnt on GCN4-ZIP will be used to produce a photo-controlled variant of the longer DNA-binding GCN4-bZIP. A different way of controlling activity will be tested by the direct control of the helicity of the basic (DNA binding) helix of MyoD, a BHLH protein.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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