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Network of molecular interactions that control the initiation of sporulation in Bacillus subtilis
Reference
B15900
Principal Investigator / Supervisor
Dr M Yudkin
Co-Investigators /
Co-Supervisors
Professor Iain Campbell
Institution
University of Oxford
Department
Biochemistry
Funding type
Research
Value (£)
187,256
Status
Completed
Type
Research Grant
Start date
01/12/2001
End date
31/01/2006
Duration
50 months
Abstract
Sporulation in Bacillus subtilis is initiated by activation of a specific transcription factor, sigmaF, which is controlled by a network of interactions amongst three proteins, SpoIIAA (AA), SpoIIAB (AB) and SpoIIE (IIE), and ATP and ADP. A detailed model of this network has recently been described. The structure of AA is known, but not those of AB or IIE. We propose NMR studies to identify the residues of AB involved in the various interactions, fluorescence experiments to confirm a postulated change in structure in AB, mutagenesis of AB to find variants with altered binding properties, the crystallisation of IIE and its fragments, and structural studies of AA in it phosphorylated form. Mathematical modelling of the network is also proposed.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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