BBSRC Portfolio Analyser
Award details
Structural studies on nitrate metabolising enzymes of Escherichia coli.
Reference
B15827
Principal Investigator / Supervisor
Professor So Iwata
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
220,128
Status
Completed
Type
Research Grant
Start date
01/02/2002
End date
31/07/2005
Duration
42 months
Abstract
Formate dehydrogenase N (Fdh-N) and nitrate reductase (Nar) are the essential components of the nitrate respiration in E. coli under anaerobic conditions. We have already purified these two membrane proteins and have obtained good crystals of Fdh-N diffracting up to 1.6 Angstrom. This proposal is to solve the structures of these two enzymes. Fdh-N is one of the largest membrane proteins so far crystallised (trimer of 180kDa monomers). A very high resolution structure of the enzyme would provide essential information to study the architecture of membrane protein complexes. The structures of Fdh-N and Nar could also reveal a detailed proton translocation mechanism by these enzymes and how this interesting organism adapts to environmental change.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search