Award details

Production and assembly of nuclear receptor/ coregulator complexes for structural studies

ReferenceB15780
Principal Investigator / Supervisor Professor Roderick Eliot Hubbard
Co-Investigators /
Co-Supervisors
Dr Igor Bronstein
Institution University of York
DepartmentChemistry
Funding typeResearch
Value (£) 266,412
StatusCompleted
TypeResearch Grant
Start date 01/06/2002
End date 31/05/2005
Duration36 months

Abstract

Ligand-inducible nuclear receptors are sequence-specific transcription factors that play a key role in gene expression. Interactions with different coactivators facilitate chromatin remodelling and communication with the basal transcriptional apparatus. Structural studies will be essential to understand the differential recruitment of coactivators by receptors in different ligand and cellular contexts. This project will develop and apply methods to overexpress, purify and characterise the oestrogen receptor and its complex with a co- activator protein, TIF2 to provide material for crystallisation and structural studies. The methods developed are a starting point for future studies of other large multi-protein complexes that mediate gene expression.

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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