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The use of chemical analogues of acetyl coenzyme A to selectivity label the substrates of transcriptional acetylases
Reference
B15674
Principal Investigator / Supervisor
Dr Eleni Stylianou
Co-Investigators /
Co-Supervisors
Professor Neil Thomas
Institution
University of Nottingham
Department
Sch of Biomedical Sciences
Funding type
Research
Value (£)
229,864
Status
Completed
Type
Research Grant
Start date
19/11/2001
End date
18/10/2004
Duration
35 months
Abstract
The acetylation of proteins by transcriptional coactivators with intrinsic acetyltransferase activity is central to the regulation of gene transcription. The aim of the project is to identify new, specific substrates (acetyl-acceptor molecules) of the transcriptional acetylase PCAF. Complementary synthesis of analogues of acetyl coenzyme A and mutants of PCAF will be designed so that the radio-labelled modified CoA will uniquely interact with the mutated PCAF acetyl-transferase only and transfer its acetyl group in a reaction mediated exclusively by this and no other acetyl transferase. Hence only the acetyl-acceptor substrates of PCAF acetyl- transferase would be radio-labelled in vivo allowing them to be identified.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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