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Directed evolution of sialic aldolases: a combinatorial approach to sialic acid mimetics

Reference	B15651
Principal Investigator / Supervisor	Professor Alan Berry
Co-Investigators / Co-Supervisors	Professor Adam Nelson
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	191,656
Status	Completed
Type	Research Grant
Start date	01/12/2001
End date	01/12/2004
Duration	36 months

Abstract

Sialic acid is involved in many important cellular recognition events and intervention by sialic acid analogues holds the key to gaining insights into these processes as well as developing treatments for diseases ranging from influenza to cancer and the development of new antibiotics. Directed evolution using DNA shuffling has the ability to scan large regions of sequence space to enable improved or novel activities to be generated. This technology will be used to produce sialic acid aldolases with broader substrate specificity than the wild-type and/or enzymes which catalyse reactions with unnatural stereoselectivity. The specificity of the resulting biocatalysts will be determined and the catalysts will be exploited in the synthesis of inhibitors of influenza virus A sialidase and other sialic acid mimetics.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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