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Is DnaI the helicase loader in the Gram +ve Bacillus?
Reference
B15519
Principal Investigator / Supervisor
Professor Panos Soultanas
Co-Investigators /
Co-Supervisors
Institution
University of Nottingham
Department
Sch of Chemistry
Funding type
Research
Value (£)
187,544
Status
Completed
Type
Research Grant
Start date
15/10/2001
End date
15/10/2004
Duration
36 months
Abstract
In E. coli there are two well characterised primosomal assembly processes, the PriA and DnaA-mediated cascades. Bacillus has both PriA and DnaA, as well as the helicase and primase proteins but it does not appear to code for homologues of the other E. coli primosomal proteins, PriB, PriC, DnaT and DnaC (helicase loader). This project will investigate the role of the putative Bacillus primosomal protein DnaI in primosome assembly. A detailed biochemical and structural characterisation will be carried out and its interaction with the hexameric replicative helicase, DnaB will be studied. Additional functional interactions of DnaI with other proteins will also be identified using yeast two-hybrid technology. This work will enhance understanding of primosomal cascades in the more clinically relevant Gram +ve bacteria.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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