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Novel mass spectrometry approaches to examine the structure and function of ribosomes

Reference	B15445
Principal Investigator / Supervisor	Professor Carol Robinson
Co-Investigators / Co-Supervisors	Dr Paola Fucini
Institution	University of Cambridge
Department	Chemistry
Funding type	Research
Value (£)	340,552
Status	Completed
Type	Research Grant
Start date	01/10/2001
End date	31/12/2004
Duration	39 months

Abstract

The enormous impact of the X-ray analysis of the ribosomal subunits from Haloarcula marismortui and Thermus thermophilus is the culmination of 35 years of extensive work. This provides an excellent starting point to address the conformational changes that accompany the various functional states of the ribosome during its cycle. To address these changes we will apply MS techniques developed to capture transient states. Specifically we plan to: (i) Examine the conformational changes that occur in the presence of elongation factors and the nascent chain through controlled dissociation of the intact particle and hydrogen exchange methodologies. (ii) Use MS to enrich samples of the ribosome carrying the nascent chain for subsequent analysis by cryo-EM. (iii) Monitor protein synthesis in real-time at the level of single ribosome assemblies. (iv) Examine the yeast ribosome.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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