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Award details
Crystallisation of the eurokaryotic mRNA export complex
Reference
B15264
Principal Investigator / Supervisor
Professor Stuart Wilson
Co-Investigators /
Co-Supervisors
Professor David Rice
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
200,032
Status
Completed
Type
Research Grant
Start date
07/01/2002
End date
06/09/2003
Duration
20 months
Abstract
The nucleocytoplasmic transport of mRNA in higher eukaryotes involves a large complex of proteins including Tap, p15, REF, various nucleoporins, E1BAP5, hGLE2, DEK, Y14 and Ran. We have developed expression/purification systems for the core components of this complex. We will initiate crystallisation trials with the core complex and develop expression systems for additional components of the complex. Using the assembled core complex we will identify a high affinity RNA substrate for crystallisation experiments and develop high level expression/purifications systems for this RNA molecule. Cocrystallisation of the core complex with other components will be attempted as they are produced in the project.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
Associated awards:
BBS/X/00093 Crystallisation of the eurokaryotic MRNA export complex
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