Award details

Structure and assembly of the pore-forming toxin hemolysin E using x-ray crystallography and electron microscopy

Reference	B15229
Principal Investigator / Supervisor	Professor Peter Artymiuk
Co-Investigators / Co-Supervisors	Professor Per Bullough, Professor J Green
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	339,652
Status	Completed
Type	Research Grant
Start date	01/05/2001
End date	30/06/2005
Duration	50 months

Abstract

The recent structure determination of the E. coli toxin haemolysin E (HlyE) has revealed the architecture of the solution form of a novel family of pore-forming toxins, and preliminary electron microscopy studies have given insights into the arrangement of the transmembrane pores, leading to the proposal of a novel mode of protein:membrane interaction (Wallace et al (2000), Cell 100: 265-276). This project will investigate the function and assembly of the HlyE assembly in the membrane, with wide implications for other membrane proteins, and determine the structure of HlyE in the membrane pore using a powerful combination of X-ray crystallography and electron cryo-microscopy (EM).

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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