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Structural studies of a novel protein translocase
Reference
B14749
Principal Investigator / Supervisor
Professor Benjamin Berks
Co-Investigators /
Co-Supervisors
Professor Andrew Hemmings
,
Professor Tracy Palmer
,
Professor Helen Saibil
Institution
University of Oxford
Department
Biochemistry
Funding type
Research
Value (£)
344,016
Status
Completed
Type
Research Grant
Start date
01/01/2002
End date
31/07/2006
Duration
55 months
Abstract
The Tat system is a recently-discovered bacterial protein export pathway. This system has the remarkable ability of being able to transport folded polypeptides across the cytoplasmic membrane without rendering the membrane ion-permeable. The Tat preprotein translocase of Escherichia coli is an approximately 600kDa integral membrane complex containing an estimated 50 polypeptide chains. Building on our recent success in imaging a Tat complex by negative strain EM we aim to (1) analyse the Tat complex by single particle EM methods (2) further develop our overexpression and purification strategies to enable a concerted attempt to grow 2D and 3D crystals of the Tat complex suitable for medium to high resolution structure determination.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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