Award details

Fluorescence fluctuation spectroscopy: probing the conformational dynamics of DNA and proteins

Reference	B14737
Principal Investigator / Supervisor	Professor David Klenerman
Co-Investigators / Co-Supervisors	Professor Sir Shankar Balasubramanian, Dr Liming Ying
Institution	University of Cambridge
Department	Chemistry
Funding type	Research
Value (£)	251,072
Status	Completed
Type	Research Grant
Start date	01/02/2001
End date	30/06/2004
Duration	41 months

Abstract

We have developed a novel method to probe the dynamics and range of conformations of DNA and proteins. The method is based on measuring the fluctuations in time or amplitude in the fluorescence resonance energy transfer between a donor and acceptor fluorophore, with less than 10 molecules in the probe volume. Building on the last grant from the BBSRC BMS Committee we will use this novel method to study the motion of the single strand overhang of a hairpin of DNA as a function of the length of the overhang and then conformational changes that Klenow polymerase undergoes during the incorporation of a nucleotide into a template primer. The method can then be applied to probe conformational changes in a wide range of systems.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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