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An integrated expression suite solution to the production of recombinant proteins including membrane proteins
Reference
B14732
Principal Investigator / Supervisor
Professor Ian Jones
Co-Investigators /
Co-Supervisors
Institution
University of Reading
Department
Animal and Microbial Sciences
Funding type
Research
Value (£)
477,876
Status
Completed
Type
Research Grant
Start date
01/11/2001
End date
30/04/2006
Duration
54 months
Abstract
Although several protein expression systems exist, the choice for any given protein is often made on empirical grounds, an initial system fails and a second is assessed and so on. Any single system is unlikely to provide the optimal expression solution for all protein types but I suggest that an inter-linked set of procedures, an expression suite, could be developed that puts system choice on sound scientific grounds. To do this, I propose to compare and contrast the performance of several protein/system combinations. The analysis will use new combined expression vectors, novel vector modifications and automated yield analysis. These experiments will develop optimised systems for the rapid recovery of high yield bioactive material.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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