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Mechanistic studies of Fe2+ oxidation and polynuclear Fe3+ oxide formation by a subunit dimer and wild-type 24-mer bacterioferritin

Reference	B14704
Principal Investigator / Supervisor	Professor Geoffrey Robert Moore
Co-Investigators / Co-Supervisors	Professor Nicolas Le Brun, Dr S Spiro
Institution	University of East Anglia
Department	Chemistry
Funding type	Research
Value (£)	202,740
Status	Completed
Type	Research Grant
Start date	01/01/2001
End date	01/01/2004
Duration	36 months

Abstract

Mechanism(s) of Fe2+ oxidation by the 24 subunit bacterioferritin (BFR) will be investigated by conventional and rapid freeze-quench or stopped-flow studies using UV-visible, EPR and 57Fe Mossbauer spectroscopies, together with EXAFS and oximetry investigations. A goal is determining the role of the dinuclear iron ferroxidase centres of BFR. Site-directed mutagenesis will be used to construct variants of BFR to test mechanistic hypotheses. Both subunit dimer forms that do not associate into 24-mers and BFR with altered ferroxidase centres will be investigated. A secondary aim is to investigate the formation of polynuclear iron species by subunit dimers that may mirror core nucleation of the 24-mer.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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