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Molecular mechanism of the bacteriolytic E protein from bacteriophage phiX174
Reference
B14699
Principal Investigator / Supervisor
Professor Timothy Bugg
Co-Investigators /
Co-Supervisors
Institution
University of Warwick
Department
Chemistry
Funding type
Research
Value (£)
166,244
Status
Completed
Type
Research Grant
Start date
01/11/2001
End date
30/04/2005
Duration
42 months
Abstract
The E genes of bacteriophage phiX174 is responsible for bacterial cell lysis. Recent genetic experiments have established that the cellular target for the 91-amino acid protein is phospho-MurNAc-pentapeptide translocase (MraY), an integral membrane protein involved in the biosynthesis of bacterial petidoglycan cell wall. The proposed research will investigate the molecular basis of the interaction between E, MraY, and a peptidyl-prolyl isomerase SlyD. Purified E will be characterised, its interaction with SlyB investigated using circular dichroism and fluorescence spectroscopy, and the interaction with MraY investigated using fluorescence spectroscopy and surface plasmon resonance.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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