Award details

Probing RNA recognition and cleavage by a key 5' end dependent endoribonuclease

Reference	B14694
Principal Investigator / Supervisor	Dr Kenneth McDowall
Co-Investigators / Co-Supervisors	Professor Jane Grasby
Institution	University of Leeds
Department	Inst of Integrative & Comparative Biolog
Funding type	Research
Value (£)	214,056
Status	Completed
Type	Research Grant
Start date	01/12/2001
End date	01/12/2004
Duration	36 months

Abstract

Although progress has been made in identifying factors that affect RNA cleavage by RNaseE, there is no mechanistic understanding of how they combine to control the rate of catalysis. Moreover, there is no description of the essential features of the cleavage reaction and the sites within RNaseE that bring about RNA recognition and cleavage remain to be determined. The purpose of this proposal is therefore to (i) determined the kinetic parameters that are affected by substrate binding and by the 5'-end phosphorylation status and sequence of substrate, (ii) characterise the kinetics of cleavage by mutants that increase RNaseE activity in vivo, (iii) attempt to identify the catalytic and 5' end sensing sites within RNaseE, and (iv) investigate further the molecular nature of RNA cleavage by RNaseE including its stereochemical course.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search