Award details

Combinatorial gene libraries for real-time elucidation of non-covalent interactions between macromolecules

Reference	B14245
Principal Investigator / Supervisor	Professor Anna Hine
Co-Investigators / Co-Supervisors	Professor D Billington, Dr Andrew James Sutherland
Institution	Aston University
Department	Sch of Life and Health Sciences
Funding type	Research
Value (£)	305,472
Status	Completed
Type	Research Grant
Start date	10/05/2001
End date	09/01/2005
Duration	44 months

Abstract

The purpose of the proposed work is to manufacture a generic technique for probing non-covalent interactions between biological macromolecules. As a model system, zinc finger proteins will be studied, but future generic application would also encompass other molecular scaffold proteins and the non-covalent interactions of random peptides. The proposal centres on the construction and subsequent assay of combinatorial gene libraries and has two distinct areas: i). Novel methodology for construction of gene libraries. ii). Design of gene libraries to eliminate conventional screening. Part i). eliminates codon redundancy within randomised gene libraries, while part ii). eliminates the requirement for biopanning, once those libraries have been expressed.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search