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Structure of alpha-latrotoxin in the membrane pore and in the complex with a G protein-coupled receptor latrophilin

Reference	B14085
Principal Investigator / Supervisor	Professor Yuri Ushkaryov
Co-Investigators / Co-Supervisors	Professor Elena Orlova, Professor Marin Van Heel
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	209,676
Status	Completed
Type	Research Grant
Start date	01/10/2000
End date	01/10/2003
Duration	36 months

Abstract

Alpha-Latrotoxin (LTX) causes massive exocytosis in secretory cells by forming membrane pores and by stimulating latrophilin, a G protein-coupled receptor. Single-particle cryo-electron microscopy (cryo-EM) will be used to study the structure of soluble forms of LTX and its complexes with latrophilin (detergent- extracted from brain or expressed as a soluble mutant). To improve the resolution of LTX structure (determined previously at approximatley14 Angstrom) and trace the protein chain, 2D crystals of LTX will be studied. Using cryo-EM we will determine the structure of the LTX pore in the membrane of empty or receptor-loaded liposomes. This work will help to understand the structure/function relationship of LTX and components of the exocytotic machinery.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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