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Heparan sulphate analogue libraries: creation using chemo-enzymatic strategies and bioactivity screening in FGF signalling

Reference	B14080
Principal Investigator / Supervisor	Professor Jeremy Turnbull
Co-Investigators / Co-Supervisors	Dr Edwin Yates
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	205,372
Status	Completed
Type	Research Grant
Start date	01/11/2000
End date	01/11/2003
Duration	36 months

Abstract

Specific sequences in heparan sulphate (HS) determine the selective binding and regulatory properties of its interaction with proteins (e.g. growth factors). Analyses of sequence-activity relationships have begun to reveal the biological roles of HS, but are hampered by the amount of HS available from natural sources. We propose to construct a library of chemically-modified polysaccharides from readily-available heparin, and generate from these a structurally-diverse array of HS saccharide analogues using enzymatic depolymerisation. Convergent screening strategies will be employed to identify active species and will be applied to the study of HS-sequence specific regulation of the prototypical FGF-FGFR signalling system. This technology will offer a powerful new approach for revealing the structure-activity relationships of HS- protein interactions.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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