Award details

Molecular characterisation of siglec-7 and its interaction with novel synthetic sialic acid based inhibitors

Reference	B14010
Principal Investigator / Supervisor	Professor Paul Crocker
Co-Investigators / Co-Supervisors	Dr Andrei Nikolaev, Professor Daan van Aalten
Institution	University of Dundee
Department	College of Life Sciences
Funding type	Research
Value (£)	178,440
Status	Completed
Type	Research Grant
Start date	01/12/2000
End date	01/05/2004
Duration	41 months

Abstract

The structural basis for carbohydrate recognition by siglec-7 and related siglecs of the immune system will be investigated. Siglec-7 and sub-domains will be crystallised in the presence and absence of carbohydrate ligands. Using structure-guided site-directed mutagenesis, residues will be identified that are important in the novel specificity of siglec-7. Surface plasmon resonance on Biacore will be performed to obtain real time binding kinetics with a range of oligosaccharide ligands. A specific siglec-7 inhibitor and rationally designed derivatives will be synthesised, together with a novel panel of sialic acid derivatives that will be used in inhibition assays, biological studies and co- crystallisation experiments with siglecs-1 and -7.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search