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The mechanisms of 8-amino-7-oxononoate and 78-diaminopelargate synthases; the first two enzymes of the biotin pathway

Reference	B13955
Principal Investigator / Supervisor	Professor Robert Baxter
Co-Investigators / Co-Supervisors	Professor Dominic Campopiano, Professor Lindsay Sawyer
Institution	University of Edinburgh
Department	Sch of Chemistry
Funding type	Research
Value (£)	225,128
Status	Completed
Type	Research Grant
Start date	01/03/2001
End date	30/06/2004
Duration	40 months

Abstract

The mechanisms of 8-amino-7-oxononoate synthase (AONS) and 7,8-diaminopelargate synthase (DAPAS) will be investigated using a combination of kinetic (steady state, stopped-flow and quenched-flow techniques), structural (X-ray) and chemical (intermediate identification & intermediate analogue syntheses) approaches. For AONS, the mechanism of the substrate aldimine, pimeloyl CoA binding, condensation, decarboxylation, product quininoid reprotonation and product release steps will be examined using wild-type and mutant proteins. A similar approach will be used for DAPAS; the chemical identity of the SAM- derived transamination product will be identified and both kinetic and structural studies will be carried out to elucidate the mechanism.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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