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Understanding how xylan-binding carbohydrate binding modules exhibit both selective and relaxed ligand specificity

Reference	B13790
Principal Investigator / Supervisor	Professor Harry Gilbert
Co-Investigators / Co-Supervisors	Professor Gideon Davies, Professor Michael Williamson
Institution	Newcastle University
Department	Agriculture Food and Rural Development
Funding type	Research
Value (£)	212,034
Status	Completed
Type	Research Grant
Start date	01/11/2000
End date	30/03/2004
Duration	41 months

Abstract

The primary objective of this project is to use xylan-binding carbohydrate binding modules (X-CBMs) as model proteins to dissect the molecular basis for protein-carbohydrate recognition. X-CBMs exhibit ligand selectivity, in that they bind specifically to beta1,4-linked xylose polymers within the complex polysaccharide matrix that comprises the plant cell wall, but also exhibit sufficient flexibility to accommodate the residues that decorate the xylan backbone. Resolution of the structural basis for the ligand specificity of these proteins will provide important insights into both the specificity and flexibility of protein-carbohydrate molecular recognition. To achieve these objectives we will solve the three dimensional structure of five X-CBMs in the presence and absence of linear and decorated ligands, and use a rational design approach to investigate the functional importance of key residues that interact with the different ligands.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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