Award details

Understanding the assembly of an anaerobic fungal cellulase/hemicellulase complex

Reference	B13766
Principal Investigator / Supervisor	Professor Michael Williamson
Co-Investigators / Co-Supervisors	Professor Harry Gilbert
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	280,820
Status	Completed
Type	Research Grant
Start date	15/01/2001
End date	15/08/2004
Duration	43 months

Abstract

Anaerobic fungi have a unique system for degrading plant cell walls, consisting of a non-catalytic scaffolding protein to which the hydrolytic enzymes are bound. The aim is to characterise this system in molecular detail. We shall: 1) isolate, sequence and express the gene for the fungal scaffolding protein and its constituent receptor domains; 2) express constructs containing 1, 2 or 3 tandem docking domains; 3) determine the structures of both receptor and docking domains and characterise interdomain interactions; 4) measure the binding of one domain to the other and characterise the structural interactions; and 5) construct a model of the resultant complex, testing our ideas using site- directed mutagenesis. We shall characterise any synergistic or competitive interactions. We request two post-docs: a structural biologist in Sheffield and a molecular biologist in Newcastle.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search