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EM and X-ray structural analyses of the eukaryotic initiation factor complex eIF4F and associated proteins

Reference	B13532
Principal Investigator / Supervisor	Professor John McCarthy
Co-Investigators / Co-Supervisors	Professor Robert Ford, Dr Marina Ptushkina, Professor David Stuart
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	357,188
Status	Completed
Type	Research Grant
Start date	02/01/2001
End date	15/03/2004
Duration	38 months

Abstract

The cap-binding complex eIF4F plays a central role in eukaryotic translation initiation by mediating the association of 40S ribosomal subunits to mRNA. It also interacts with the Poly(A) binding protein PAB, thus facilitating communication between the 5' and 3' ends of the mRNA. In this project we will: 1) assemble eIF4F complexes using recombinant translation factors whose biological activities have been tested using biochemical assays; 2) perform EM analysis of the eIF4F complex and or derivative complexes at the single particle level and via the generation of 2D crystal arrays; and 3) generate crystals of these complexes and determine high resolution structures via X-ray diffraction.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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