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Sperm-egg recognition: high sensitivity mass spectrometric characterisation of murine zona pellucida glycoproteins

Reference	B13433
Principal Investigator / Supervisor	Professor Anne Dell
Co-Investigators / Co-Supervisors	Professor H Morris
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	187,180
Status	Completed
Type	Research Grant
Start date	01/06/2000
End date	01/06/2003
Duration	36 months

Abstract

Evidence collected during the past two decades indicates that murine sperm-egg binding is dependent upon the recognition of oligosaccharides that coat the zona pellucida (mZP). Several models have been proposed to mediate this interaction, but the structures of the molecules involved remain enigmatic. Preliminary work from our laboratory has recently provided the first evidence for the presence of the Sda antigen in mZP. We are seeking funding to continue these studies with the objective of rigorously characterising the glycosylation profile of each of the three murine ZP glycoproteins (mZP1, mZP2 and mZP3). This research will be complemented by structural studies of mZP from transgenic mice expressing the human ZP3 gene. This work will lead to a better understanding of carbohydrate-protein recognition in the fertilisation process.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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