Award details

Assembled complexes of eukaryotic and archaeal RNA polymerase subunits: a structural NMR investigation

Reference	B13343
Principal Investigator / Supervisor	Professor Steve Matthews
Co-Investigators / Co-Supervisors	Dr Robert Weinzierl
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	152,844
Status	Completed
Type	Research Grant
Start date	01/02/2001
End date	01/02/2004
Duration	36 months

Abstract

DNA-dependent RNA polymerases (RNAPs) play a fundamental role in the gene expression program of every organism. Eukaryotic and archaeal RNAPs are very complex enzymes consisting of several subunits. Studying high-resolution structures of intact eukaryotic RNA polymerase by diffraction methods is generally inhibited by the ability to produce high quality crystals. We propose to use NMR spectroscopy to determine structures of RNAP subunit assemblages in solution with particular emphasis being placed on complexes, which are likely to make important contributions to catalytic activities and regulatory functions. This work will have implications both in biotechnology and the targeted development of drugs.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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