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Structural basis for transcriptional regulation by the NMRA & AREA proteins

Reference	B13335
Principal Investigator / Supervisor	Professor Alastair Hawkins
Co-Investigators / Co-Supervisors	Dr Heather Lamb, Professor David Stammers
Institution	Newcastle University
Department	School of Cell and Molecular Biosciences
Funding type	Research
Value (£)	266,812
Status	Completed
Type	Research Grant
Start date	01/11/2000
End date	26/02/2004
Duration	40 months

Abstract

In ascomycetous fungi the nitrogen metabolite repression signal transduction pathway (STP) controls the expression of over a hundred genes in an array of nitrogen utilisation pathways, and the AREA protein plays a key role within this STP. We have shown that the NMRA protein binds tightly to the C-terminal region of AREA in vitro implying that it may post- translationally modulate the biological activity of AREA in vivo. We propose 1) to determine the structure of the NMRA protein; 2) to attempt determination of the structure of NMRA with the C- terminal region of AREA; and 3) to use site directed mutagenesis and surface plasmon resonance measurements to determine the regions of AREA essential for the interaction with NMRA.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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