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Structure of a eukaryotic small heat shock protein by high resolution single particle electron microscopy
Reference
B12984
Principal Investigator / Supervisor
Professor Helen Saibil
Co-Investigators /
Co-Supervisors
Dr Helen White
Institution
Birkbeck College
Department
Biological Sciences
Funding type
Research
Value (£)
240,945
Status
Completed
Type
Research Grant
Start date
01/01/2000
End date
01/01/2003
Duration
36 months
Abstract
Small heat shock proteins, a superfamily of molecular chaperones including the eye lens protein alpha-crystallin, form large and variable macromolecular assemblies. They play a protective role, suppressing protein aggregation. We propose to determine the structure of two assemblies of yeast hsp26 by high resolution single particle analysis of electron cryo- micrographs. Our preliminary work has revealed hollow shells with octahedral or tetrahedral symmetry. Under heat stress, the structure is recognised to form much larger assemblies with denatured substrate proteins. We will also characterise the structures of hsp26-substrate complexes. This work should reveal the structural basis of variable assembly and substrate binding in small hsps.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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