BBSRC Portfolio Analyser
Award details
Membrane translocation of a toxin endonuclease
Reference
B12974
Principal Investigator / Supervisor
Professor Colin Kleanthous
Co-Investigators /
Co-Supervisors
Institution
University of York
Department
Biology
Funding type
Research
Value (£)
198,311
Status
Completed
Type
Research Grant
Start date
01/10/2000
End date
30/09/2003
Duration
36 months
Abstract
The endonuclease domain from the bacterial toxin colicin E9 can translocate across membranes in the absence of energy or receptor proteins. Using bilayer experiments, unilamellar vesicles (in combination with a range of biophysical techniques) and the crystal structure of the DNase to design mutants we will: 1) Optimise the conditions for membrane translocation. 2) Investigate the properties of DNase channels. 3) Examine the effect of immunity protein binding on membrane translocations. 4) Address the link between DNase conformational stability and membrane translocation. 5) Define protein structural changes associated with membrane binding. 6) Investigate protein-membrane interactions and protein import into liposomes.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search