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Structural characterisation of the reg proteins and their molecular interactions

Reference	B12958
Principal Investigator / Supervisor	Dr Mary Phillips-Jones
Co-Investigators / Co-Supervisors	Professor Peter Henderson, Professor Simon Phillips, Professor Michael Williamson
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	152,708
Status	Completed
Type	Research Grant
Start date	01/04/2000
End date	01/04/2003
Duration	36 months

Abstract

We aim to initiate a detailed structural characterisation of the Reg two- component signal transduction proteins (RegA and RegB), and their interactions with other pathway components, in order to determine how photosynthesis, CO2- and N2-fixation are differentially regulated by the Reg pathway in response to oxygen in Rhodobacter sphaeroides. Purified RegA, RegB and other components will be amplified in E. coli, and subjected to a range of genetic, biophysical and structural methods: (i) to determine the activity, structure and structural changes of these proteins in isolation, upon phosphorylation, and in interactions with their known ligands/proteins; (ii) to obtain the first quantitative data on RegA-RegB interactions; (iii) to identify the Reg-A binding site(s) in the target promoter DNA of photosynthesis genes; and (iv) to identify which of the proposed signals is actually sensed by RegB. The proposed studies, which uniquely involve full length proteins, will also contribute to understanding of two-component function in general (and ultimately to rational drug design).

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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