Award details

Atomic force microscopy of multi-subunit protein assemblies

Reference	B12816
Principal Investigator / Supervisor	Dr Robert Henderson
Co-Investigators / Co-Supervisors	Professor John Edwardson
Institution	University of Cambridge
Department	Pharmacology
Funding type	Research
Value (£)	196,877
Status	Completed
Type	Research Grant
Start date	01/01/2000
End date	01/01/2003
Duration	36 months

Abstract

We will use atomic force microscopy to study two macromolecular assemblies. The first is a protein complex which is known to mediate membrane fusion. The complex will be constructed in a supported lipid bilayer, and the response of its structure to known triggers of membrane fusion will be examined. In particular we will look for the formation of higher- order structures which might represent analogues of the proposed 'fusion-pore'. In parallel experiments we will investigate the subunit composition of members of the superfamily of ligand-gated ion channels, including the GABAa, and nicotinic acetylcholine receptors. We will focus on the arrangement of the subunits that constitute the pentameric quaternary structure of these complexes.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search