Award details

Assembly and structure of a membrane protein supercomplex

Reference	B12745
Principal Investigator / Supervisor	Professor Per Bullough
Co-Investigators / Co-Supervisors	Krebs Professor of B Christopher Hunter
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	281,508
Status	Completed
Type	Research Grant
Start date	24/01/2001
End date	24/01/2006
Duration	60 months

Abstract

We will apply a combination of molecular genetics, biochemistry, spectroscopy, electron and X-ray crystallography to study the molecular architecture and factors determining assembly of very large membrane-bound protein complexes. We have identified an excellent model system; the photosynthetic membrane of Rhodobacter sphaeroides which is made up of a complex arrangement of macromolecular assemblies. We will identify the important factors in determining the interactions between different components of the membrane. We will obtain structures of components of the system using cryo-EM or X-ray crystallography, including wild-type and mutant reaction centre/antenna complexes. An integral membrane protein essential for correct assembly of the photosynthetic apparatus will be overexpressed for structural studies.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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