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Expression purification and crystallisation of the herpes simplex virus (HSV) virulence factor ICP34.5
Reference
B12733
Principal Investigator / Supervisor
Dr Andrew A. Freer
Co-Investigators /
Co-Supervisors
Professor S Brown
Institution
University of Glasgow
Department
School of Chemistry
Funding type
Research
Value (£)
83,148
Status
Completed
Type
Research Grant
Start date
01/03/2000
End date
03/10/2002
Duration
31 months
Abstract
The RL1 gene of HSV encodes the protein ICP34.5 which is a specific virulence determinant. ICP34.5 functions by complexing through a 63 amino acid conserved domain with proliferating cell nuclear antigen, PCNA. Two homologous cellular proteins, MyD116 and GADD34, also interact with PCNA. To define precisely the sites of interaction between ICP34.5 and PCNA, we aim to (i) overexpress ICP34.5, the 63 amino acid domain and other constructs of IPC34.5; (ii) overexpress the 63 amino acid domains of MyD116 and GADD34; (iii) purify the expressed proteins using glutathione sepharose, ion exchange and gel filtration FPLC; and (iv) set up crystallisation trials on either the whole ICP34.5 or its truncations.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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