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Design construction and characterisation of hybrids between Zn-binding and BHLH-domains
Reference
B12712
Principal Investigator / Supervisor
Professor Rudolf Allemann
Co-Investigators /
Co-Supervisors
Institution
University of Birmingham
Department
School of Chemistry
Funding type
Research
Value (£)
165,392
Status
Completed
Type
Research Grant
Start date
01/03/2000
End date
01/08/2003
Duration
41 months
Abstract
The exquisite specificity of transcriptional initiation in higher organisms is in sharp contrast to the often modest DNA binding specificity of eukaryotic transcriptional regulators in vitro. For specific DNA recognition many transcription factors rely on protein-protein interactions. To test the hypothesis that high specificity of DNA binding depends on reduced flexibility of the DNA recognition motif, we propose to synthesise mutants of the developmentally important BHLH-proteins MyoD and MASH-1, in which the basic recognition helix is stabilised through a Zn2+ binding domain. A thorough biochemical and biophysical characterisation of the hybrids will be performed. These hybrids will provide us with a model to study the effects of protein-protein interactions and intramolecular stabilisation on the DNA binding specificity of BHLH-proteins.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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