Award details

Probing the mechanism of ribosomal peptidyl transferase and esterase activity

Reference	B12055
Principal Investigator / Supervisor	Professor Rudolf Allemann
Co-Investigators / Co-Supervisors	Professor Martin Ryan
Institution	University of Birmingham
Department	School of Chemistry
Funding type	Research
Value (£)	120,827
Status	Completed
Type	Research Grant
Start date	01/01/2000
End date	01/01/2003
Duration	36 months

Abstract

We have shown that the aphtho- and cardiovirus 2A-mediated polyprotein cleavage is not a proteolytic reaction, but rather a novel manipulation of the translational apparatus. In addition we have shown this mechanism to be a property of other, non-picorna-, virus and cellular sequences. We wish to investigate the mechanism of this reaction using a combination of molecular biological and synthetic organic chemical techniques. This research program will not only give us insights into how 2A interacts with the peptidyltransferase centre of the ribosome but will enable us to use a unique probe into the events and structures involved in peptide bond formation. The proposed program of work leads us into new lines of enquiry, but builds upon both: (a) complementary expertise; (b) already established reagents, techniques and collaborations. (Joint with award B11989.)

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

B11989 Probing the mechanism of ribosomal peptidyl transferase and esterase activity

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