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Mechanistic studies of a 5-prime nuclease

Reference	B11990
Principal Investigator / Supervisor	Professor Jane Grasby
Co-Investigators / Co-Supervisors	Professor Jon Sayers
Institution	University of Sheffield
Department	Chemistry
Funding type	Research
Value (£)	176,837
Status	Completed
Type	Research Grant
Start date	07/02/2000
End date	07/02/2003
Duration	36 months

Abstract

5-prime nuclease are required for efficient DNA replication in organisms as diverse as bacteriophage and humans yet the mechanistic details of this family of enzymes remain obscure. We plan to study the mechanism of a phage member of this family employing a novel single cleavage assay for the enzyme. These experiments will involve a combination of pre-steady state kinetics, stereochemical elucidation and modified DNA substrates to act as mechanistic and structural probes. The studies are designed to elucidate the role of metal ions in the reaction, to investigate the potential of nucleophilic catalysis and to confirm the juxtaposition of and electrostatic catalyst and the scissile bond.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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