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In vivo/in vitro immunisation and phage display: which is the best method for generating catalytic antibodies?
Reference
B11968
Principal Investigator / Supervisor
Professor Neil Thomas
Co-Investigators /
Co-Supervisors
Institution
University of Nottingham
Department
Sch of Chemistry
Funding type
Research
Value (£)
220,020
Status
Completed
Type
Research Grant
Start date
28/02/2000
End date
28/04/2003
Duration
38 months
Abstract
The aim of this research is to evaluate a number of antibody generation methods to determine the relative advantages of each method in the creation of catalytic antibodies. Whilst the majority of catalytic antibodies reported in the literature have been produced using in vivo immunisation followed by preparation of monoclonals using hybridomas, several other approaches are now available. Using haptens that have generated well characterised catalysis for both hydrolytic and pericyclic rearrangement reactions we will survey the options currently available. These include in vitro immunisation, preparation of both immunised and naive phage display libraries and the generation of high affinity ovine antibodies. This research will indicate the limitations of the various approaches and also produce information on whether a specific antibody production approach favours either a specific antibody subset or type of catalytic mechanism.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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