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The mechanism of action of lignin peroxidase: towards designer substrate oxidation sites
Reference
B11934
Principal Investigator / Supervisor
Professor Andrew Trevor Smith
Co-Investigators /
Co-Supervisors
Dr Christopher Davies
,
Dr Wendy Doyle
Institution
University of Sussex
Department
Sch of Life Sciences
Funding type
Research
Value (£)
190,592
Status
Completed
Type
Research Grant
Start date
01/09/1999
End date
01/01/2003
Duration
40 months
Abstract
Understanding of the catalytic mechanism of lignin peroxidase has been greatly facilitated by the discovery of a unique substrate oxidation site involving the redox active side chain of W171 and long-range electron transfer to the haem. We will: (i) investigate the role of the negatively charged environment of W171 in the oxidation of veratryl alcohol (ii), test a putative electron transfer pathway to the haem and (iii) investigate the role of residues controlling the access of larger substrates to the classical haem-edge substrate interaction site. Physical and kinetic studies of specific mutants will be complemented by their structure determination by X-ray crystallography.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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