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A genetic biochemical and crystallographic investigation of an interaction between a membrane protein and cardiolipin
Reference
B11801
Principal Investigator / Supervisor
Professor Mike Jones
Co-Investigators /
Co-Supervisors
Professor Neil William Isaacs
Institution
University of Bristol
Department
Biochemistry
Funding type
Research
Value (£)
51,744
Status
Completed
Type
Research Grant
Start date
01/11/1999
End date
01/11/2000
Duration
12 months
Abstract
A combination of site directed mutagenesis, biochemical treatments and X-ray crystallography will be used to investigate possible functional roles of a specific interaction that has been identified between an integral membrane protein, the bacterial photoreaction centre, and the diacidic phospholipid cardiolipin. Two conserved basic residues that bind the phosphate head groups of the cardiolipin will be mutated to neutral residues, and the effect on assembly and function of the reaction centre will be examined. Caliometry will be used to examine the effect of cardiolipin binding on stability of the reaction centre. X-ray crystallography will be used to examine the effect of mutations on cardiolipin binding by the reaction centre.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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