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Development of rupture force spectroscopy to probe molecular recognition
Reference
B11764
Principal Investigator / Supervisor
Professor David Klenerman
Co-Investigators /
Co-Supervisors
Professor Chris Abell
,
Dr Feydor Dultsev
Institution
University of Cambridge
Department
Chemistry
Funding type
Research
Value (£)
166,518
Status
Completed
Type
Research Grant
Start date
04/08/1999
End date
30/01/2003
Duration
42 months
Abstract
We seek funds to develop a new method to probe molecular recognition by measuring the rupture force required to separate the ligand and receptor. This will be done by increasing the amplitude of oscillation of a quartz-crystal microbalance (QCM) until bond breakage is detected. We have performed proof of concept experiments and demonstrated we can break the streptavidin/biotin bond and will extend these experiments so measurements can be made reliably in solution. We will perform quantitative measurements of avidin/biotin, enzyme/substrates, antibody/antigen and DNA hybridisation in solution. We will also explore the use of this new technique as a new form of separation science based on varying the strength of molecular interaction by varying the amplitude of oscillation of the QCM.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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