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Structure function and mechanism of UDP-galactomutase

ReferenceB11756
Principal Investigator / Supervisor Professor James Naismith
Co-Investigators /
Co-Supervisors
Institution University of St Andrews
DepartmentBiology
Funding typeResearch
Value (£) 168,052
StatusCompleted
TypeResearch Grant
Start date 01/02/2000
End date 31/10/2003
Duration45 months

Abstract

UDP-galactopyranose mutase is an enzyme responsible for the conversion of UDP- galactose pyranose (six membered ring) into UDP- galactose furanose (five membered ring). This mechanistically fascinating transformation is accomplished using two co-factors FAD and NAD(P)H. The role of these co-factors is unknown. UDP- galactofuranose is a key component in cell walls of many pathogenic bacteria including Mycobacteria tuberculosis and Klebsiella pneumoniae. We propose to solve the structure of this enzyme from three organisms and determine the mechanism. We have obtained diffraction data from one source (E. coli), crystals from a second (K. pneumoniae) and expressed protein from the third (M. tuberculosis).

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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