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The nature and mechanism of coupled and gated redox reactions of iron-sulphur centres in proteins
Reference
B11675
Principal Investigator / Supervisor
Professor Fraser Armstrong
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Oxford Chemistry
Funding type
Research
Value (£)
158,145
Status
Completed
Type
Research Grant
Start date
01/12/1999
End date
01/12/2002
Duration
36 months
Abstract
The research will develop further a new and powerful technique to observe and analyse coupled/gated electron-transfer reactions in biological systems, and will examine aspects crucial to the action of both redox-driven proton pumps and Fe-S active sites. Fast scan protein-film voltammetry, supported by stopped-flow kinetics, spectroscopy and molecular biology methods, will be used to study the combined kinetics and energetics of coupled redox reactions of Fe-S clusters, down to time scales greater than 10-3 sec. These include (1) Proton binding at [3Fe-4S]o, and a detailed study of long-range proton transfer to the buried cluster in the crystallographically defined Azotobacter vinelandii ferredoxin and mutant forms, and (2) Unusual reactivities of all-Fe(II) [3Fe-4S]2- clusters, which can undergo very rapid, reversible, 2e/2H+ oxidation likely to involve disulphide-coupling.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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