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Investigating RNA-RNA RNA-antibiotic and RNA-polyamine interactions exploiting the neurospora VS ribozyme

Reference	B11652
Principal Investigator / Supervisor	Professor Jane Grasby
Co-Investigators / Co-Supervisors	Dr Iain Ashmore Murray
Institution	University of Sheffield
Department	Chemistry
Funding type	Research
Value (£)	175,406
Status	Completed
Type	Research Grant
Start date	18/11/1999
End date	18/11/2002
Duration	36 months

Abstract

The Neurospora VS ribozyme is an example of a catalytic RNA molecule. This ribozyme differs from other small ribozymes in that it recognises a substrate with inherent secondary structure, a stem loop. We plan to study the details of the substrate ribozyme interaction as a paradigm for RNA-RNA interactions. We will attempt to set up a fluorescent assay for ribozymic action. We will probe enzyme substrate contacts at the functional group level. Further more, the ribozyme is stimulated by both polyamines and an antibiotic, and we will also use mutant substrates to define interaction with these molecules. We will also map the binding site of the substrate on the ribozyme.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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