Award details

Forced evolution of class II aldolases: new reagents for organic synthesis

Reference	B11596
Principal Investigator / Supervisor	Professor Alan Berry
Co-Investigators / Co-Supervisors	Professor Adam Nelson
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	173,940
Status	Completed
Type	Research Grant
Start date	01/11/1999
End date	01/01/2003
Duration	38 months

Abstract

Enzymes, such as the aldolases, offer uniquely powerful opportunities for the stereospecific formation of carbon-carbon bonds in organic synthesis. Their use for the production of novel sugars has met with limited success, however, because of the inherent substrate specificity of the natural enzymes. This project will tackle this issue by developing a novel assay using chromogenic substrates or reagents and forced evolution to generate large libraries of aldolases with new enzyme activities. The structure-activity relationship of the new enzymes will not only provide important fundamental insights into how aldolases achieve their powerful catalysis, but could provide new synthetic routes for novel biologically active sugar of commercial importance.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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